Abstract: Objective C3 gene over-expression mice were constructed and bred to identify and analyse their genotypes and the expression of target proteins, and to verify the applicability of the assay. Method The constructed C3 gene over-expression positive founder mice were bred with wild-type mice, and the heterozygous mice were obtained by sibling mating ( male-to-female ratio of 1 ∶ 1 ) , and the pure heterozygous mice were obtained. The genotypes of heterozygotes and heterozygotes were identified by PCR of mouse tail tissue DNA, and the differences in C3 protein expression in brain tissue between mice were detected by Western blot. The body weight changes and reproductive performance of heterozygous and pure heterozygous C3 over-expressing mice were analyzed. Result C3 gene over-expression heterozygous mice could obtain pure heterozygous mice by sibling mating. Protein blotting showed that C3 protein was highly expressed in the tissues of transgenic mice compared with wild-type mice, and the over-expression of C3 gene did not affect the reproduction of mice, and the mating was stable among the homozygous mice. However, over-expression of the C3 gene significantly reduced the body weight of transgenic female mice after 5 weeks of age. Conclusion The C3 over-expression mice can be stably obtained by full-sib mating and genotyping by PCR.

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